ANTIBACTERIAL AND ANTIVIRAL ACTIVITIES OF STINGING NETTLE (URTICA DIOICA L.) LEAF EXTRACT ON NOROVIRUS AND CAMPYLOBACTER JEJUNI AS FOODBORNE PATHOGENS

Research background. The cytotoxicity on various vital cell lines and the activity foodborne pathogens (murine norovirus 1 as a surrogate Campylobacter jejuni) of methanolic Urtica dioica L. leaf extract (UDE) were studied. Experimental approach. cytotoxic concentration 50% (CC50) was measured by linearity between UDE concentrations viability. Antibacterial effects C. jejuni analyzed broth microdilution method with spectrophotometer. virucidal antiviral activities determined virus titration host infectivity expressed tissue infective dose using Spearman–Karber. Results conclusions. CC50 macrophage cell. MIC MBC 5 mg/ml 20 for both isolated from poultry meat standard strain. inhibited MNV-1 three pathways at approximately same inhibitory (1.45-1.87 mg/ml). In conclusion, present study tried to explain in detail dose-dependent two important causing outbreaks worldwide. results showed that it might be safe alternative food additive supplement candidate concentrations.